《植物生理学报》 2012, 48(10): 958-964

甘蓝型油菜叶表皮蜡质组分及结构与菌核病抗性关系

王婧*, 刘泓利*, 宋超, 倪郁**

西南大学农学与生物科技学院, 重庆 400716

通信作者：倪郁；E-mail: nmniyu@126.com；Tel: 023-68251264

摘　要：

本试验选用6个抗病性不同的甘蓝型油菜品种, 研究其叶表皮蜡质组成及结构与菌核病抗性的关系。结果表明, 抗病 品种在去除叶表皮蜡质后病情指数显著增加; 感病品种无显著变化。不同抗性品种(系)间除酯类组分含量无显著差异外, 其余蜡质组分含量差异显著。相关分析表明, 蜡质组分中酯类含量与病情指数呈显著负相关关系, 醇类、酮类含量与病情 指数呈显著正相关, 其余组分和蜡质总量与病情指数无显著相关关系。抗性品种叶表皮蜡质中烷类及酯类所占比重较高, 而易感品种酮类比重较高。扫描电镜结果显示, 抗病品种(系)的蜡质晶体主要为颗粒状、杆状、丝状; 而感病品种(系)的 蜡质晶体中不规则片状晶体所占比例较大。这些结果说明油菜叶表皮蜡质的组分及结构可能是抗病品种抵抗和延迟病原 菌侵入的机制之一。

关键词：甘蓝型油菜; 表皮蜡质; 抗性; 菌核病

收稿：2012-09-07   修定：2012-09-29

资助：国家自然科学基金(31000122)、重庆市科委自然科学基金计划(cstc2012jjA80022)和中央高校基本科研业务费专项资金(XDJK2011C007)。

Relationship between Brassica napus Epicuticular Wax Composition and Structure and Resistance to Sclerotinia sclerotiorum

WANG Jing*, LIU Hong-Li*, SONG Chao, NI Yu**

College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China

Corresponding author: WANG Jing; E-mail: nmniyu@126.com; Tel: 023-68251264

Abstract:

Six cultivars of Brassica napus with different resistance to Sclerotinia sclerotiorum were selected to investigate the composition and crystalloid structure of leaf epicuticular. The results showed that the disease index increased significantly in the resistant cultivars after the surface wax was removed, while no significant change was observed for the susceptible cultivars. The contents of wax constituents differed significantly among cultivars except for esters. Correlation analysis indicated that the disease index was negatively correlated with the contents of esters, positively correlated with the contents of alcohols and ketones, insignificantly correlated with the contents of other wax constituents and total wax. The weight proportions of alkanes and esters in total waxes of the resistant cultivars were higher than those of the susceptible cultivars, while the weight proportion of ketones in total waxes of the susceptible cultivars was higher than that of the resistant cultivars. The SEM analysis showed that crystalloid structure of epicuticular waxes on leaves of the resistant cultivars mainly included granulars, rods and filamentous, while more plates were appeared on leaves of the susceptible cultivars. These results suggested that the constituents of the wax and their crystalloid structure might be one of the mechanisms for resistant cultivars to resist and delay the pathogen invasion.

Key words: Brassica napus; epicuticular wax; resistance; Sclerotinia sclerotiorum